A single amino acid substitution in PRKDC is a determinant of sensitivity to Adriamycin-induced renal injury in mouse

Adriamycin (ADR)-induced nephropathy is frequently utilized in rodent models of podocytopathy. However, the application this model mice limited to a few strains, such as BALB/c mice. The most commonly used mouse strain, C57BL/6 (B6), resistant ADR-induced nephropathy, are all strains with B6 genetic background. Reportedly, R2140C variant Prkdc gene cause susceptibility To verify hypothesis, we produced mutant mice, termed B6-PrkdcR2140C, that possess mutation. After administration ADR, B6-PrkdcR2140C exhibited massive proteinuria and glomerular renal tubular injuries. In addition, there was no significant difference severity between BALB/c. These findings demonstrated show at similar level BALB/c, PRKDC causes nephropathy. future studies, may become applicable various kinds genetically modified background by mating B6-PrkdcR2140C.